Multiplexion - Cell Line Authentication

Authentication of human cell lines by STR profiling

For cell lines not included in our SNP typing data base, we recommend human cell line authentication using STR profiling
Analysis of the following markers: CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, vWA, and the gender marker Amelogenin
Includes all relevant markers of ANSI/ATCC ASN0002-2011. Authentication of human cell lines: Standardization of STR profiling
Authentication of newly-established human cell lines or sublines by genotyping paired donor and derived cell line samples

Purify cell line DNA by commercially available DNA purification systems, e.g. from Qiagen or Roche (protocol)
- required DNA concentration: 15 ng/µL
- required amount: >40 µL
- tubes: 1.5 ml SafeLock tubes (please do not use screw caps and Parafilm)
Complete the
Label sample tubes with "STR" and the specific sample name as provided in the confirmation Email
Ship samples in a padded envelope WITH a copy of the confirmation Email to:

Multiplexion, c/o DKFZ, Dept. D320, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

STR profiling of submitted cell line(s)
Comparison of obtained signature to reference signatures at DSMZ, ATCC (optionally).
Chromatogram of the DNA profile as PDF (optionally).
Transfer of results to customer via Email (the report can be used as official certificate for granting agencies and peer-reviewed journals)

Usually 5-10 working days upon receipt of samples.

Customers from Heidelberg can drop off samples in the building INF242 between 9 AM and 12 AM each Friday.

Different contaminations require different actions. An overview can be found here.
A database of misidentified or cross-contaminated cell lines can be found here. Please stop working with any of these cell lines.
Problems with contaminations? Our check list for sterile work may help.

Please note: This service is FOR RESEARCH USE ONLY. Do not use in diagnostic procedures.